high sensitivity elisa kits Search Results


sensitive elisa kits  (FUJIFILM)
90
FUJIFILM sensitive elisa kits
Cholesterol depletion <t>reduces</t> <t>Aβ,</t> APP CTFs and cell surface APP without affecting AICD generation. A) PS70 or HEK cells were treated with 20 μM lovastatin in the presence or absence of DLFBS for 48 hours and Aβ levels were measured by sandwich <t>ELISA.</t> Treatment with lovastatin led to a reduction of Aβ40 and Aβ42 that is prevented by the presence of cholesterol-containing serum. B) PS70 cells were treated with lovastatin or MβCDX in the presence or absence of DLFBS for 48 hours and membrane preparations were obtained as described (Sastre et al. 2001). Levels of full-length APP and APP CTFs were measured by Western blot. Cholesterol depletion reduced both α- and β-CTFs that can be recovered by addition of cholesterol-containing serum. Quantification from four independent experiments is shown below. C) HEK cells were transfected with BAP-APP construct and treated with vehicle, lovastatin, 5 or 10 mM MβCDX in FBS or DLFBS. Cell surface APP was biotinylated and the cell lysate was subjected to Western blot analysis for APP expression. Treatment with lovastatin or/and MβCDX reduced cell surface APP without altering total APP expression. D) Cell-free AICD generation assay. PS70 cells were treated with different concentrations of MβCDX and membrane preparations were incubated at 37 °C for 2 hours. Extent of cholesterol reduction is indicated below. APP CTFs and AICD were measured by Western blot. No changes in AICD generation were observed after cholesterol depletion. Incubation at 4 °C or with DAPT inhibited the production of AICD. Average data from three independent experiments are shown below. E) Aβ1-x levels were measured in the same cell-free assay and results were normalized to levels of APP CTFs. Only a slight decrease was observed at the highest MβCDX concentration. Incubation at 4°C and more markedly treatment with DAPT reduced Aβ generation. Data represent the average of two independent experiments.
Sensitive Elisa Kits, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+sensitivity+elisa+kits/pmc02741735-180-10-13?v=FUJIFILM
Average 90 stars, based on 1 article reviews
sensitive elisa kits - by Bioz Stars, 2026-08
90/100 stars
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high-sensitivity commercial enzyme-linked immunosorbent assay kits zymutest hs-crp  (DiaPharma Group Inc)
90
DiaPharma Group Inc high-sensitivity commercial enzyme-linked immunosorbent assay kits zymutest hs-crp
Cholesterol depletion <t>reduces</t> <t>Aβ,</t> APP CTFs and cell surface APP without affecting AICD generation. A) PS70 or HEK cells were treated with 20 μM lovastatin in the presence or absence of DLFBS for 48 hours and Aβ levels were measured by sandwich <t>ELISA.</t> Treatment with lovastatin led to a reduction of Aβ40 and Aβ42 that is prevented by the presence of cholesterol-containing serum. B) PS70 cells were treated with lovastatin or MβCDX in the presence or absence of DLFBS for 48 hours and membrane preparations were obtained as described (Sastre et al. 2001). Levels of full-length APP and APP CTFs were measured by Western blot. Cholesterol depletion reduced both α- and β-CTFs that can be recovered by addition of cholesterol-containing serum. Quantification from four independent experiments is shown below. C) HEK cells were transfected with BAP-APP construct and treated with vehicle, lovastatin, 5 or 10 mM MβCDX in FBS or DLFBS. Cell surface APP was biotinylated and the cell lysate was subjected to Western blot analysis for APP expression. Treatment with lovastatin or/and MβCDX reduced cell surface APP without altering total APP expression. D) Cell-free AICD generation assay. PS70 cells were treated with different concentrations of MβCDX and membrane preparations were incubated at 37 °C for 2 hours. Extent of cholesterol reduction is indicated below. APP CTFs and AICD were measured by Western blot. No changes in AICD generation were observed after cholesterol depletion. Incubation at 4 °C or with DAPT inhibited the production of AICD. Average data from three independent experiments are shown below. E) Aβ1-x levels were measured in the same cell-free assay and results were normalized to levels of APP CTFs. Only a slight decrease was observed at the highest MβCDX concentration. Incubation at 4°C and more markedly treatment with DAPT reduced Aβ generation. Data represent the average of two independent experiments.
High Sensitivity Commercial Enzyme Linked Immunosorbent Assay Kits Zymutest Hs Crp, supplied by DiaPharma Group Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+sensitivity+elisa+kits/10__1097_slash_psy__0000000000000344-83-6-19?v=DiaPharma+Group+Inc
Average 90 stars, based on 1 article reviews
high-sensitivity commercial enzyme-linked immunosorbent assay kits zymutest hs-crp - by Bioz Stars, 2026-08
90/100 stars
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high-sensitivity crp elisa  (BQ Kits Inc)
90
BQ Kits Inc high-sensitivity crp elisa
Cholesterol depletion <t>reduces</t> <t>Aβ,</t> APP CTFs and cell surface APP without affecting AICD generation. A) PS70 or HEK cells were treated with 20 μM lovastatin in the presence or absence of DLFBS for 48 hours and Aβ levels were measured by sandwich <t>ELISA.</t> Treatment with lovastatin led to a reduction of Aβ40 and Aβ42 that is prevented by the presence of cholesterol-containing serum. B) PS70 cells were treated with lovastatin or MβCDX in the presence or absence of DLFBS for 48 hours and membrane preparations were obtained as described (Sastre et al. 2001). Levels of full-length APP and APP CTFs were measured by Western blot. Cholesterol depletion reduced both α- and β-CTFs that can be recovered by addition of cholesterol-containing serum. Quantification from four independent experiments is shown below. C) HEK cells were transfected with BAP-APP construct and treated with vehicle, lovastatin, 5 or 10 mM MβCDX in FBS or DLFBS. Cell surface APP was biotinylated and the cell lysate was subjected to Western blot analysis for APP expression. Treatment with lovastatin or/and MβCDX reduced cell surface APP without altering total APP expression. D) Cell-free AICD generation assay. PS70 cells were treated with different concentrations of MβCDX and membrane preparations were incubated at 37 °C for 2 hours. Extent of cholesterol reduction is indicated below. APP CTFs and AICD were measured by Western blot. No changes in AICD generation were observed after cholesterol depletion. Incubation at 4 °C or with DAPT inhibited the production of AICD. Average data from three independent experiments are shown below. E) Aβ1-x levels were measured in the same cell-free assay and results were normalized to levels of APP CTFs. Only a slight decrease was observed at the highest MβCDX concentration. Incubation at 4°C and more markedly treatment with DAPT reduced Aβ generation. Data represent the average of two independent experiments.
High Sensitivity Crp Elisa, supplied by BQ Kits Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+sensitivity+elisa+kits/pm26872295-145-17-20?v=BQ+Kits+Inc
Average 90 stars, based on 1 article reviews
high-sensitivity crp elisa - by Bioz Stars, 2026-08
90/100 stars
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rat aqp-2 elisa kit  (MyBiosource Biotechnology)
90
MyBiosource Biotechnology rat aqp-2 elisa kit
Cholesterol depletion <t>reduces</t> <t>Aβ,</t> APP CTFs and cell surface APP without affecting AICD generation. A) PS70 or HEK cells were treated with 20 μM lovastatin in the presence or absence of DLFBS for 48 hours and Aβ levels were measured by sandwich <t>ELISA.</t> Treatment with lovastatin led to a reduction of Aβ40 and Aβ42 that is prevented by the presence of cholesterol-containing serum. B) PS70 cells were treated with lovastatin or MβCDX in the presence or absence of DLFBS for 48 hours and membrane preparations were obtained as described (Sastre et al. 2001). Levels of full-length APP and APP CTFs were measured by Western blot. Cholesterol depletion reduced both α- and β-CTFs that can be recovered by addition of cholesterol-containing serum. Quantification from four independent experiments is shown below. C) HEK cells were transfected with BAP-APP construct and treated with vehicle, lovastatin, 5 or 10 mM MβCDX in FBS or DLFBS. Cell surface APP was biotinylated and the cell lysate was subjected to Western blot analysis for APP expression. Treatment with lovastatin or/and MβCDX reduced cell surface APP without altering total APP expression. D) Cell-free AICD generation assay. PS70 cells were treated with different concentrations of MβCDX and membrane preparations were incubated at 37 °C for 2 hours. Extent of cholesterol reduction is indicated below. APP CTFs and AICD were measured by Western blot. No changes in AICD generation were observed after cholesterol depletion. Incubation at 4 °C or with DAPT inhibited the production of AICD. Average data from three independent experiments are shown below. E) Aβ1-x levels were measured in the same cell-free assay and results were normalized to levels of APP CTFs. Only a slight decrease was observed at the highest MβCDX concentration. Incubation at 4°C and more markedly treatment with DAPT reduced Aβ generation. Data represent the average of two independent experiments.
Rat Aqp 2 Elisa Kit, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+sensitivity+elisa+kits/10__1128_slash_iai__00264___17-155-21-30?v=MyBiosource+Biotechnology
Average 90 stars, based on 1 article reviews
rat aqp-2 elisa kit - by Bioz Stars, 2026-08
90/100 stars
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high-sensitivity sandwich elisa kits for hs-crp  (DPC Cirrus Inc)
90
DPC Cirrus Inc high-sensitivity sandwich elisa kits for hs-crp
Cholesterol depletion <t>reduces</t> <t>Aβ,</t> APP CTFs and cell surface APP without affecting AICD generation. A) PS70 or HEK cells were treated with 20 μM lovastatin in the presence or absence of DLFBS for 48 hours and Aβ levels were measured by sandwich <t>ELISA.</t> Treatment with lovastatin led to a reduction of Aβ40 and Aβ42 that is prevented by the presence of cholesterol-containing serum. B) PS70 cells were treated with lovastatin or MβCDX in the presence or absence of DLFBS for 48 hours and membrane preparations were obtained as described (Sastre et al. 2001). Levels of full-length APP and APP CTFs were measured by Western blot. Cholesterol depletion reduced both α- and β-CTFs that can be recovered by addition of cholesterol-containing serum. Quantification from four independent experiments is shown below. C) HEK cells were transfected with BAP-APP construct and treated with vehicle, lovastatin, 5 or 10 mM MβCDX in FBS or DLFBS. Cell surface APP was biotinylated and the cell lysate was subjected to Western blot analysis for APP expression. Treatment with lovastatin or/and MβCDX reduced cell surface APP without altering total APP expression. D) Cell-free AICD generation assay. PS70 cells were treated with different concentrations of MβCDX and membrane preparations were incubated at 37 °C for 2 hours. Extent of cholesterol reduction is indicated below. APP CTFs and AICD were measured by Western blot. No changes in AICD generation were observed after cholesterol depletion. Incubation at 4 °C or with DAPT inhibited the production of AICD. Average data from three independent experiments are shown below. E) Aβ1-x levels were measured in the same cell-free assay and results were normalized to levels of APP CTFs. Only a slight decrease was observed at the highest MβCDX concentration. Incubation at 4°C and more markedly treatment with DAPT reduced Aβ generation. Data represent the average of two independent experiments.
High Sensitivity Sandwich Elisa Kits For Hs Crp, supplied by DPC Cirrus Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+sensitivity+elisa+kits/10__2147_slash_trrm__s194011-56-10-15?v=DPC+Cirrus+Inc
Average 90 stars, based on 1 article reviews
high-sensitivity sandwich elisa kits for hs-crp - by Bioz Stars, 2026-08
90/100 stars
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high-density lipoprotein (hdl) elisa kits for dogs  (MyBiosource Biotechnology)
90
MyBiosource Biotechnology high-density lipoprotein (hdl) elisa kits for dogs
Cholesterol depletion <t>reduces</t> <t>Aβ,</t> APP CTFs and cell surface APP without affecting AICD generation. A) PS70 or HEK cells were treated with 20 μM lovastatin in the presence or absence of DLFBS for 48 hours and Aβ levels were measured by sandwich <t>ELISA.</t> Treatment with lovastatin led to a reduction of Aβ40 and Aβ42 that is prevented by the presence of cholesterol-containing serum. B) PS70 cells were treated with lovastatin or MβCDX in the presence or absence of DLFBS for 48 hours and membrane preparations were obtained as described (Sastre et al. 2001). Levels of full-length APP and APP CTFs were measured by Western blot. Cholesterol depletion reduced both α- and β-CTFs that can be recovered by addition of cholesterol-containing serum. Quantification from four independent experiments is shown below. C) HEK cells were transfected with BAP-APP construct and treated with vehicle, lovastatin, 5 or 10 mM MβCDX in FBS or DLFBS. Cell surface APP was biotinylated and the cell lysate was subjected to Western blot analysis for APP expression. Treatment with lovastatin or/and MβCDX reduced cell surface APP without altering total APP expression. D) Cell-free AICD generation assay. PS70 cells were treated with different concentrations of MβCDX and membrane preparations were incubated at 37 °C for 2 hours. Extent of cholesterol reduction is indicated below. APP CTFs and AICD were measured by Western blot. No changes in AICD generation were observed after cholesterol depletion. Incubation at 4 °C or with DAPT inhibited the production of AICD. Average data from three independent experiments are shown below. E) Aβ1-x levels were measured in the same cell-free assay and results were normalized to levels of APP CTFs. Only a slight decrease was observed at the highest MβCDX concentration. Incubation at 4°C and more markedly treatment with DAPT reduced Aβ generation. Data represent the average of two independent experiments.
High Density Lipoprotein (Hdl) Elisa Kits For Dogs, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+sensitivity+elisa+kits/pmc07255649-85-15-16?v=MyBiosource+Biotechnology
Average 90 stars, based on 1 article reviews
high-density lipoprotein (hdl) elisa kits for dogs - by Bioz Stars, 2026-08
90/100 stars
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high sensitive human - amyloid elisa kits for a 42 quantification  (FUJIFILM)
90
FUJIFILM high sensitive human - amyloid elisa kits for a 42 quantification
Cholesterol depletion <t>reduces</t> <t>Aβ,</t> APP CTFs and cell surface APP without affecting AICD generation. A) PS70 or HEK cells were treated with 20 μM lovastatin in the presence or absence of DLFBS for 48 hours and Aβ levels were measured by sandwich <t>ELISA.</t> Treatment with lovastatin led to a reduction of Aβ40 and Aβ42 that is prevented by the presence of cholesterol-containing serum. B) PS70 cells were treated with lovastatin or MβCDX in the presence or absence of DLFBS for 48 hours and membrane preparations were obtained as described (Sastre et al. 2001). Levels of full-length APP and APP CTFs were measured by Western blot. Cholesterol depletion reduced both α- and β-CTFs that can be recovered by addition of cholesterol-containing serum. Quantification from four independent experiments is shown below. C) HEK cells were transfected with BAP-APP construct and treated with vehicle, lovastatin, 5 or 10 mM MβCDX in FBS or DLFBS. Cell surface APP was biotinylated and the cell lysate was subjected to Western blot analysis for APP expression. Treatment with lovastatin or/and MβCDX reduced cell surface APP without altering total APP expression. D) Cell-free AICD generation assay. PS70 cells were treated with different concentrations of MβCDX and membrane preparations were incubated at 37 °C for 2 hours. Extent of cholesterol reduction is indicated below. APP CTFs and AICD were measured by Western blot. No changes in AICD generation were observed after cholesterol depletion. Incubation at 4 °C or with DAPT inhibited the production of AICD. Average data from three independent experiments are shown below. E) Aβ1-x levels were measured in the same cell-free assay and results were normalized to levels of APP CTFs. Only a slight decrease was observed at the highest MβCDX concentration. Incubation at 4°C and more markedly treatment with DAPT reduced Aβ generation. Data represent the average of two independent experiments.
High Sensitive Human Amyloid Elisa Kits For A 42 Quantification, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+sensitivity+elisa+kits/10__3233_slash_jad___200637-102-34-37?v=FUJIFILM
Average 90 stars, based on 1 article reviews
high sensitive human - amyloid elisa kits for a 42 quantification - by Bioz Stars, 2026-08
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noradrenaline high-sensitivity elisa kit nou39-k01  (DLD Diagnostika)
90
DLD Diagnostika noradrenaline high-sensitivity elisa kit nou39-k01
Cholesterol depletion <t>reduces</t> <t>Aβ,</t> APP CTFs and cell surface APP without affecting AICD generation. A) PS70 or HEK cells were treated with 20 μM lovastatin in the presence or absence of DLFBS for 48 hours and Aβ levels were measured by sandwich <t>ELISA.</t> Treatment with lovastatin led to a reduction of Aβ40 and Aβ42 that is prevented by the presence of cholesterol-containing serum. B) PS70 cells were treated with lovastatin or MβCDX in the presence or absence of DLFBS for 48 hours and membrane preparations were obtained as described (Sastre et al. 2001). Levels of full-length APP and APP CTFs were measured by Western blot. Cholesterol depletion reduced both α- and β-CTFs that can be recovered by addition of cholesterol-containing serum. Quantification from four independent experiments is shown below. C) HEK cells were transfected with BAP-APP construct and treated with vehicle, lovastatin, 5 or 10 mM MβCDX in FBS or DLFBS. Cell surface APP was biotinylated and the cell lysate was subjected to Western blot analysis for APP expression. Treatment with lovastatin or/and MβCDX reduced cell surface APP without altering total APP expression. D) Cell-free AICD generation assay. PS70 cells were treated with different concentrations of MβCDX and membrane preparations were incubated at 37 °C for 2 hours. Extent of cholesterol reduction is indicated below. APP CTFs and AICD were measured by Western blot. No changes in AICD generation were observed after cholesterol depletion. Incubation at 4 °C or with DAPT inhibited the production of AICD. Average data from three independent experiments are shown below. E) Aβ1-x levels were measured in the same cell-free assay and results were normalized to levels of APP CTFs. Only a slight decrease was observed at the highest MβCDX concentration. Incubation at 4°C and more markedly treatment with DAPT reduced Aβ generation. Data represent the average of two independent experiments.
Noradrenaline High Sensitivity Elisa Kit Nou39 K01, supplied by DLD Diagnostika, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+sensitivity+elisa+kits/10__1172_slash_jci136542-265-15-21?v=DLD+Diagnostika
Average 90 stars, based on 1 article reviews
noradrenaline high-sensitivity elisa kit nou39-k01 - by Bioz Stars, 2026-08
90/100 stars
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elisa kits high-density lipoprotein cholesterol (hdl-c)  (CUSAg Inc)
90
CUSAg Inc elisa kits high-density lipoprotein cholesterol (hdl-c)
Cholesterol depletion <t>reduces</t> <t>Aβ,</t> APP CTFs and cell surface APP without affecting AICD generation. A) PS70 or HEK cells were treated with 20 μM lovastatin in the presence or absence of DLFBS for 48 hours and Aβ levels were measured by sandwich <t>ELISA.</t> Treatment with lovastatin led to a reduction of Aβ40 and Aβ42 that is prevented by the presence of cholesterol-containing serum. B) PS70 cells were treated with lovastatin or MβCDX in the presence or absence of DLFBS for 48 hours and membrane preparations were obtained as described (Sastre et al. 2001). Levels of full-length APP and APP CTFs were measured by Western blot. Cholesterol depletion reduced both α- and β-CTFs that can be recovered by addition of cholesterol-containing serum. Quantification from four independent experiments is shown below. C) HEK cells were transfected with BAP-APP construct and treated with vehicle, lovastatin, 5 or 10 mM MβCDX in FBS or DLFBS. Cell surface APP was biotinylated and the cell lysate was subjected to Western blot analysis for APP expression. Treatment with lovastatin or/and MβCDX reduced cell surface APP without altering total APP expression. D) Cell-free AICD generation assay. PS70 cells were treated with different concentrations of MβCDX and membrane preparations were incubated at 37 °C for 2 hours. Extent of cholesterol reduction is indicated below. APP CTFs and AICD were measured by Western blot. No changes in AICD generation were observed after cholesterol depletion. Incubation at 4 °C or with DAPT inhibited the production of AICD. Average data from three independent experiments are shown below. E) Aβ1-x levels were measured in the same cell-free assay and results were normalized to levels of APP CTFs. Only a slight decrease was observed at the highest MβCDX concentration. Incubation at 4°C and more markedly treatment with DAPT reduced Aβ generation. Data represent the average of two independent experiments.
Elisa Kits High Density Lipoprotein Cholesterol (Hdl C), supplied by CUSAg Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+sensitivity+elisa+kits/pmc10188040-87-11-19?v=CUSAg+Inc
Average 90 stars, based on 1 article reviews
elisa kits high-density lipoprotein cholesterol (hdl-c) - by Bioz Stars, 2026-08
90/100 stars
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human-ultra-sensitive custom-made 7-plex electrochemiluminescent based elisa kits  (Meso Scale Diagnostics LLC)
90
Meso Scale Diagnostics LLC human-ultra-sensitive custom-made 7-plex electrochemiluminescent based elisa kits
Cholesterol depletion <t>reduces</t> <t>Aβ,</t> APP CTFs and cell surface APP without affecting AICD generation. A) PS70 or HEK cells were treated with 20 μM lovastatin in the presence or absence of DLFBS for 48 hours and Aβ levels were measured by sandwich <t>ELISA.</t> Treatment with lovastatin led to a reduction of Aβ40 and Aβ42 that is prevented by the presence of cholesterol-containing serum. B) PS70 cells were treated with lovastatin or MβCDX in the presence or absence of DLFBS for 48 hours and membrane preparations were obtained as described (Sastre et al. 2001). Levels of full-length APP and APP CTFs were measured by Western blot. Cholesterol depletion reduced both α- and β-CTFs that can be recovered by addition of cholesterol-containing serum. Quantification from four independent experiments is shown below. C) HEK cells were transfected with BAP-APP construct and treated with vehicle, lovastatin, 5 or 10 mM MβCDX in FBS or DLFBS. Cell surface APP was biotinylated and the cell lysate was subjected to Western blot analysis for APP expression. Treatment with lovastatin or/and MβCDX reduced cell surface APP without altering total APP expression. D) Cell-free AICD generation assay. PS70 cells were treated with different concentrations of MβCDX and membrane preparations were incubated at 37 °C for 2 hours. Extent of cholesterol reduction is indicated below. APP CTFs and AICD were measured by Western blot. No changes in AICD generation were observed after cholesterol depletion. Incubation at 4 °C or with DAPT inhibited the production of AICD. Average data from three independent experiments are shown below. E) Aβ1-x levels were measured in the same cell-free assay and results were normalized to levels of APP CTFs. Only a slight decrease was observed at the highest MβCDX concentration. Incubation at 4°C and more markedly treatment with DAPT reduced Aβ generation. Data represent the average of two independent experiments.
Human Ultra Sensitive Custom Made 7 Plex Electrochemiluminescent Based Elisa Kits, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+sensitivity+elisa+kits/pmc06279219-61-8-15?v=Meso+Scale+Diagnostics+LLC
Average 90 stars, based on 1 article reviews
human-ultra-sensitive custom-made 7-plex electrochemiluminescent based elisa kits - by Bioz Stars, 2026-08
90/100 stars
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adiponectin human elisa, high sensitivity (sandwich)  (BioVendor Instruments)
92
BioVendor Instruments adiponectin human elisa, high sensitivity (sandwich)
Cholesterol depletion <t>reduces</t> <t>Aβ,</t> APP CTFs and cell surface APP without affecting AICD generation. A) PS70 or HEK cells were treated with 20 μM lovastatin in the presence or absence of DLFBS for 48 hours and Aβ levels were measured by sandwich <t>ELISA.</t> Treatment with lovastatin led to a reduction of Aβ40 and Aβ42 that is prevented by the presence of cholesterol-containing serum. B) PS70 cells were treated with lovastatin or MβCDX in the presence or absence of DLFBS for 48 hours and membrane preparations were obtained as described (Sastre et al. 2001). Levels of full-length APP and APP CTFs were measured by Western blot. Cholesterol depletion reduced both α- and β-CTFs that can be recovered by addition of cholesterol-containing serum. Quantification from four independent experiments is shown below. C) HEK cells were transfected with BAP-APP construct and treated with vehicle, lovastatin, 5 or 10 mM MβCDX in FBS or DLFBS. Cell surface APP was biotinylated and the cell lysate was subjected to Western blot analysis for APP expression. Treatment with lovastatin or/and MβCDX reduced cell surface APP without altering total APP expression. D) Cell-free AICD generation assay. PS70 cells were treated with different concentrations of MβCDX and membrane preparations were incubated at 37 °C for 2 hours. Extent of cholesterol reduction is indicated below. APP CTFs and AICD were measured by Western blot. No changes in AICD generation were observed after cholesterol depletion. Incubation at 4 °C or with DAPT inhibited the production of AICD. Average data from three independent experiments are shown below. E) Aβ1-x levels were measured in the same cell-free assay and results were normalized to levels of APP CTFs. Only a slight decrease was observed at the highest MβCDX concentration. Incubation at 4°C and more markedly treatment with DAPT reduced Aβ generation. Data represent the average of two independent experiments.
Adiponectin Human Elisa, High Sensitivity (Sandwich), supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+sensitivity+elisa+kits/custom%40rd191023100%4037686759?v=BioVendor+Instruments
Average 92 stars, based on 1 article reviews
adiponectin human elisa, high sensitivity (sandwich) - by Bioz Stars, 2026-08
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human high sensitivity c reactive protein elisa kit  (Sunlong Biotech Co Ltd)
86
Sunlong Biotech Co Ltd human high sensitivity c reactive protein elisa kit
Cholesterol depletion <t>reduces</t> <t>Aβ,</t> APP CTFs and cell surface APP without affecting AICD generation. A) PS70 or HEK cells were treated with 20 μM lovastatin in the presence or absence of DLFBS for 48 hours and Aβ levels were measured by sandwich <t>ELISA.</t> Treatment with lovastatin led to a reduction of Aβ40 and Aβ42 that is prevented by the presence of cholesterol-containing serum. B) PS70 cells were treated with lovastatin or MβCDX in the presence or absence of DLFBS for 48 hours and membrane preparations were obtained as described (Sastre et al. 2001). Levels of full-length APP and APP CTFs were measured by Western blot. Cholesterol depletion reduced both α- and β-CTFs that can be recovered by addition of cholesterol-containing serum. Quantification from four independent experiments is shown below. C) HEK cells were transfected with BAP-APP construct and treated with vehicle, lovastatin, 5 or 10 mM MβCDX in FBS or DLFBS. Cell surface APP was biotinylated and the cell lysate was subjected to Western blot analysis for APP expression. Treatment with lovastatin or/and MβCDX reduced cell surface APP without altering total APP expression. D) Cell-free AICD generation assay. PS70 cells were treated with different concentrations of MβCDX and membrane preparations were incubated at 37 °C for 2 hours. Extent of cholesterol reduction is indicated below. APP CTFs and AICD were measured by Western blot. No changes in AICD generation were observed after cholesterol depletion. Incubation at 4 °C or with DAPT inhibited the production of AICD. Average data from three independent experiments are shown below. E) Aβ1-x levels were measured in the same cell-free assay and results were normalized to levels of APP CTFs. Only a slight decrease was observed at the highest MβCDX concentration. Incubation at 4°C and more markedly treatment with DAPT reduced Aβ generation. Data represent the average of two independent experiments.
Human High Sensitivity C Reactive Protein Elisa Kit, supplied by Sunlong Biotech Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
human high sensitivity c reactive protein elisa kit - by Bioz Stars, 2026-08
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Cholesterol depletion reduces Aβ, APP CTFs and cell surface APP without affecting AICD generation. A) PS70 or HEK cells were treated with 20 μM lovastatin in the presence or absence of DLFBS for 48 hours and Aβ levels were measured by sandwich ELISA. Treatment with lovastatin led to a reduction of Aβ40 and Aβ42 that is prevented by the presence of cholesterol-containing serum. B) PS70 cells were treated with lovastatin or MβCDX in the presence or absence of DLFBS for 48 hours and membrane preparations were obtained as described (Sastre et al. 2001). Levels of full-length APP and APP CTFs were measured by Western blot. Cholesterol depletion reduced both α- and β-CTFs that can be recovered by addition of cholesterol-containing serum. Quantification from four independent experiments is shown below. C) HEK cells were transfected with BAP-APP construct and treated with vehicle, lovastatin, 5 or 10 mM MβCDX in FBS or DLFBS. Cell surface APP was biotinylated and the cell lysate was subjected to Western blot analysis for APP expression. Treatment with lovastatin or/and MβCDX reduced cell surface APP without altering total APP expression. D) Cell-free AICD generation assay. PS70 cells were treated with different concentrations of MβCDX and membrane preparations were incubated at 37 °C for 2 hours. Extent of cholesterol reduction is indicated below. APP CTFs and AICD were measured by Western blot. No changes in AICD generation were observed after cholesterol depletion. Incubation at 4 °C or with DAPT inhibited the production of AICD. Average data from three independent experiments are shown below. E) Aβ1-x levels were measured in the same cell-free assay and results were normalized to levels of APP CTFs. Only a slight decrease was observed at the highest MβCDX concentration. Incubation at 4°C and more markedly treatment with DAPT reduced Aβ generation. Data represent the average of two independent experiments.

Journal:

Article Title: MILD CHOLESTEROL DEPLETION REDUCES AMYLOID-? PRODUCTION BY IMPAIRING APP TRAFFICKING TO THE CELL SURFACE

doi: 10.1111/j.1471-4159.2009.06126.x

Figure Lengend Snippet: Cholesterol depletion reduces Aβ, APP CTFs and cell surface APP without affecting AICD generation. A) PS70 or HEK cells were treated with 20 μM lovastatin in the presence or absence of DLFBS for 48 hours and Aβ levels were measured by sandwich ELISA. Treatment with lovastatin led to a reduction of Aβ40 and Aβ42 that is prevented by the presence of cholesterol-containing serum. B) PS70 cells were treated with lovastatin or MβCDX in the presence or absence of DLFBS for 48 hours and membrane preparations were obtained as described (Sastre et al. 2001). Levels of full-length APP and APP CTFs were measured by Western blot. Cholesterol depletion reduced both α- and β-CTFs that can be recovered by addition of cholesterol-containing serum. Quantification from four independent experiments is shown below. C) HEK cells were transfected with BAP-APP construct and treated with vehicle, lovastatin, 5 or 10 mM MβCDX in FBS or DLFBS. Cell surface APP was biotinylated and the cell lysate was subjected to Western blot analysis for APP expression. Treatment with lovastatin or/and MβCDX reduced cell surface APP without altering total APP expression. D) Cell-free AICD generation assay. PS70 cells were treated with different concentrations of MβCDX and membrane preparations were incubated at 37 °C for 2 hours. Extent of cholesterol reduction is indicated below. APP CTFs and AICD were measured by Western blot. No changes in AICD generation were observed after cholesterol depletion. Incubation at 4 °C or with DAPT inhibited the production of AICD. Average data from three independent experiments are shown below. E) Aβ1-x levels were measured in the same cell-free assay and results were normalized to levels of APP CTFs. Only a slight decrease was observed at the highest MβCDX concentration. Incubation at 4°C and more markedly treatment with DAPT reduced Aβ generation. Data represent the average of two independent experiments.

Article Snippet: To measure human Aβ 1-42 and Aβ 1-x we used sensitive ELISA kits (Wako, Japan and IBL, Germany, respectively).

Techniques: Sandwich ELISA, Western Blot, Transfection, Construct, Expressing, Incubation, Cell-Free Assay, Concentration Assay